Necrotic enteritis (NE) caused by Clostridium perfringens is considered one of the most common enteric diseases, causing huge economic losses to the global poultry industry ( Wade et al., 2015). perfringens infection by inhibiting the virulence-associated traits and virulence genes expression.Įnteric diseases are of major concern to the poultry industry due to the increased mortality, impaired production and potential contamination of poultry products. This study suggests that baicalin-aluminum may be a potential candidate against C. perfringens and Escherichia coli and raised the relative abundance of Lactobacillus in the ileal digesta. Besides, baicalin-aluminum alleviated the upregulation of C. In vivo experiments, baicalin-aluminum lightened the intestinal lesions and histological damage, it inhibited pro-inflammatory cytokines (TNF-α, IL-1β, IL-6) expression in the jejunal and ileal tissues. Additionally, baicalin-aluminum was found to negatively regulate the expression of genes involved in quorum sensing (QS) communication, including genes of Agr QS system ( agrB, agrD) and genes of VirS/R two-component regulatory system ( virS, virR). The results showed that baicalin-aluminum inhibited bacterial hemolytic activity, diminished biofilm formation, attenuated cytotoxicity to Caco-2 cells, downregulated the expression of genes encoding for clostridial toxins and extracellular enzymes such as alpha toxin (CPA), perfringolysin O (PFO), collagenase (ColA), and sialidases (NanI, NanJ). perfringens CVCC2030, it also evaluated the in vivo therapeutic effect on NE. The present study investigated the effects of baicalin-aluminum on the virulence-associated traits and virulence genes expression of C. Baicalin-aluminum complex is synthesized from baicalin, a flavonoid isolated from Scutellaria baicalensis Georgi. Since the use of antibiotics in feed is withdrawn, it is imperative to find out suitable alternatives to control NE. 2Hubei Collaborative Innovation Center for Animal Nutrition and Feed Safety, Wuhan Polytechnic University, Wuhan, ChinaĬlostridium perfringens type A is the main cause of necrotic enteritis (NE) in chickens.1Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan, China.Jin Liu 1,2† Shuangqi Wu 1,2† Honghao Zhao 1,2 Chun Ye 1,2 Shulin Fu 1,2 Yu Liu 1,2 Ting Liu 1,2 Yinsheng Qiu 1,2*
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